JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for the detection of genotoxic carcinogens: I. Summary of pre-validation study results.

Report of the JaCVAM initiative international pre-validation studies of the in vivo rodent alkaline Comet assay for the detection of genotoxic carcinogens

Recent Advances in In Vivo Genotoxicity Testing: Prediction of Carcinogenic Potential Using Comet and Micronucleus Assay in Animal Models

Genotoxic events have been known as crucial step in the initiation of cancer. To assess the risk of cancer, genotoxicity assays, including comet, micronucleus (MN), chromosomal aberration, bacterial reverse, and sister chromatid exchange assay, can be performed. Compared with in vitro genotoxicity assay, in vivo genotoxicity assay has been used to verify in vitro assay result and definitely pro...

Collaborative study on fifteen compounds in the rat-liver Comet assay integrated into 2- and 4-week repeat-dose studies.

A collaborative trial was conducted to evaluate the possibility of integrating the rat-liver Comet assay into repeat-dose toxicity studies. Fourteen laboratories from Europe, Japan and the USA tested fifteen chemicals. Two chemicals had been previously shown to induce micronuclei in an acute protocol, but were found negative in a 4-week Micronucleus (MN) Assay (benzo[a]pyrene and 1,2-dimethylhy...

Genotoxic and Antigenotoxic Activity of Silymarin by an In Vivo Bone Marrow Micronucleus Assay and Comet Assay

Introduction: Silymarin is obtained from Silybum marianum (milk thistle), an edible plant that has long been used medicinally for the treatment of liver-related disorders. Silymarin is a powerful hepatoprotective and antioxidant but the anticlastogenic activity, which is an important aspect of its cancer chemoprevention is not known hence the present investigation was carried out to study its...

Positive effects of Vitamin C in arsenic trioxide and sodium fluoride induced genotoxicity and oxidative stress in mice in vivo

The aim of the present study was to evaluate Vitamin C (VC) as a potent natural antioxidant to mitigate the genotoxic effects of Arsenic trioxide and sodium fluoride in Swiss albino mice in vivo. The study was divided into eight groups consisting of control treated with normal saline (Group I), Group II, III, IV, V, VI, VII and the VC group with only Vitamin C (500 mg/kgbw). Arsenic trioxide (4...